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Varioskan LUX Multimode Microplate Reader

MANUFACTURER Thermo Fisher Scientific (manufacturer's website)
CONTACT 1 David Moir
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Description

Catering to all applications, skill sets Varioskan LUX is a versatile tool for busy labs. Configure the instrument to your needs, then upgrade when your research focus changes. Supports the following measurement technologies: • Absorbance (UV-Vis, including pathlength correction) • Fluorescence Intensity (including FRET) • Luminescence (direct and filtered, including BRET) • AlphaScreen / AlphaLISA • Time-resolved fluorescence (including TR-FRET, hTRF) Flexible wavelength selection The instrument selects the measurement wavelength using filters or monochromators, depending on which is optimal for each measurement technology. • Monochromators in absorbance and fluorescence intensity • Filters in AlphaScreen and time-resolved fluorescence • Luminescence without wavelength selection (filters can be used if required) The instrument also allows spectral scanning for ultimate flexibility for identifying the optimal measurement wavelength for any assay, now and in the future. Features Automatic dynamic range selection Many multimode readers require the user to adjust PMT gain voltages manually in order to achieve the optimal sample signals for fluorescence and luminescence assays. But this manual trial and error process takes time, and can result in loss of sensitivity or over-saturation of signals. The Varioskan LUX multimode reader has an automatic dynamic range selection feature designed to automatically select the optimal reading range based on the signal intensity in each well - easing assay setup and helping you get optimal results, every time. Smart safety controls The potential for experimental errors—from user mistakes to power outages—are a frustrating reality of microplate reading. But the Varioskan LUX instrument is designed with automatic safety checks designed to alert you of potential problems before they happen. Integrated gas control Some cell-based assays require precise control over carbon dioxide and oxygen concentrations to keep the atmosphere optimal for cell growth. The ability to control O2 and CO2 levels in your microplate reader replaces the need for constant handling of plates between the incubator and reader, and helps to ensure that cells remain alive throughout the experiment.

Specification

Absorbance

Plate types 6, 384 well plates Wavelength selection Double monochromators Wavelength range 200–1,000 nm Light source Xenon flash lamp Read out range 0–6 Abs Linear measurement range 0–4 Abs (96-well plate) at 450 nm, ± 2% 0–3 Abs (384-well plate) at 450 nm, ± 2% Accuracy 0.003 Abs or ± 2%, at 200–399 nm (0–2 Abs) 0.003 Abs or ± 1%, at 400–1,000 nm (0–3 Abs) Precision SD <0.001 Abs or CV <0.5%, at 450 nm (0–3 Abs)

Fluorescence Intensity

Plate types 6–1,536 well plates Wavelength selection Double excitation and emission monochromators Excitation wavelength range 200–1,000 nm Emission wavelength range 270–840 nm Light source Xenon flash lamp Sensitivity Top reading: <0.4 fmol fluorescein/well (black 384-well plate) Bottom reading: <4 fmol fluorescein/well, (clear bottom black 384 square well plate) Dynamic range Top reading >6 decades Bottom reading >5.5 decades

Time-resolved fluorescence

Plate types 6–1,536 well plates Wavelength selection Filters (spectral scanning with double excitation and emission monochromators) Excitation wavelength range Fixed to 334 nm (spectral scanning 200–840 nm) Emission wavelength range 400–700 nm (spectral scanning 270–840 nm) Light source Xenon flash lamp Sensitivity <1 amol Eu/well (white low volume 384 well plate) Dynamic range >6 decades

Luminescence

Plate types 6–1,536 well plates (spectral scanning 6, 384 well plates) Wavelength selection Direct or filters (spectral scanning with double monochromators) Wavelength range 360–670 nm Sensitivity <7 amol ATP/well (white 384-well plate) Dynamic range >7 decades

AlphaScreen

Plate types 6, 1,536 well plates Wavelength selection Filters Excitation wavelength range Fixed to 680 nm Emission wavelength range 400–660 nm Light source LED Sensitivity <100 amol phosphotyrosine/well (white 384-well plate)

Dispensing

Plate types 6, 384 well plates No. of dispensers None, one or two Syringe size 1 ml (standard), 5 ml (optional) Dispensing volume 2–5,000 μl, in 1 µl increments (1 ml syringe) 5–25,000 μl, in 5 µl increments (5 ml syringe) Accuracy <1 μl with 50 μl (0.4 mm tip) <0.2 μl with 5 μl (0.25 mm tip) Precision <1 μl with 50 μl (0.4 mm tip) <0.25 μl with 5 μl (0.25 mm tip) Dead volume Reagent loss <100 μl, Total tubing volume <800 μl

Incubator and Shaker

Temperature range From ambient + 4°C to 45 °C Shaking type Orbital

Integrated Gas Module

CO2 concentration range 0.1–15% CO2 concentration stability ± 0.3% at 5% CO2 O2 concentration range 1–21 % O2 concentration stability ±0.3% at 1% O2

General Features

Measurement modes End-point, kinetic, spectra, multipoint and kinetic spectra Measurement speed Reads a 96-well plate in 15 s, a 384-well plate in 45 s, and a 1,536-well plate in 135 s (minimum times) Interface PC software (SkanIt software) Dimensions (D x W x H) 58 x 53 x 51 cm (23 x 21 x 20 in) Weight 54–59 kg (119–130 lb), depending on configuration

Item ID #1566.

Last Updated: 6th April, 2017