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Superdex Peptide

MANUFACTURER GE Healthcare
MODEL 10/300 GL
CONTACT 1 Rebecca Kenyon
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Description

Superdex Peptide 10/300 GL is a prepacked column for high-resolution size exclusion chromatography for small-scale preparative purification and analysis of peptides and other small biomolecules with molecular weights between 100 and 7000. • High-resolution separations of proteins, peptides, and other biomolecules according to size. • Well suited for the polishing step in a purification procedure. • Easy and predictable scale-up. • Excellent reproducibility and durability.

Superdex prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. High performance gel filtration offers fast separations with high resolution for a variety of applications including protein purification, studies of complex formation, and screening of uncharacterized samples.

Specification

Bed Dimensions 10 mm × N/A Includes Valco fittings for ÄKTAdesign systems and are supplied with M6 connectors for FPLC System. Bed Volume 24 ml Sample Volume ≤250 µl Complete Packsize 1 Piece Storage Conditions 4 to 30°C, 20% Ethanol Pressure Max. [Over the Packed Bed During Operation] 18 bar [1.8 MPa] (261 psi) Order Information Not suitable for use with ÄKTAprime plus system. Theoretical Plates > 25000 m-1

Media

Particle size, d50V 13 µm1) Matrix Spherical composite of cross-linked agarose and dextran pH Stability Working Range 1–14 pH stability, CIP 1–14 pH Stability Cleaning 1–14 Storage Conditions 4 to 30°C, 20% Ethanol Chemical Stability Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning-in-place)

1)d50v is the median particle size of the cumulative volume distribution.

Column

Column i.d. 10 mm Bed Dimensions 10 mm × N/A Complete Packsize 1 Piece Chemical Stability Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons Material [Column Hardware] Borosilicate Glass, Polyetheretherketone (PEEK), Polypropylene (PP), Ethylene Propylene Diene Monomer (EPDM) and Perfluoro-rubber (PFR) Material [Column Tube] Borosilicate Glass Material [Bed Support] Polypropylene (PP) filter Bed Support Filter Porosity 7 μm

Item ID #1565.

Last Updated: 7th April, 2017