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LSRFORTESSA X-20

MANUFACTURER Becton Dickinson (manufacturer's website)
CONTACT 1 Debra Jones
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Description

The BD LSRFortessa X-20 is designed to support your current assay requirements. In addition, as future needs arise, optical capability can be added or upgraded.

A compact footprint and height of 76.2 x 73.7 x 76.2 cm (30 x 29 x 30 in.) allows the BD LSRFortessa X-20 to fit easily on the benchtop for the cost-effective use of valuable laboratory space. In addition to the reduced size, design enhancements provide easier access to bandpass filters and mirrors, simplifying changes in the instrument configuration.

To improve experimental workflow, the optional BD™ High Throughput Sampler (HTS) provides rapid, fully automated sample acquisition from microtiter plates. In high-throughput mode, the HTS option can speed through a 96-well plate in less than 15 minutes with less than 0.5% sample carryover from one well to the next. Low carryover is essential to ensure data quality in research applications.

Specification

Optics Excitation Optics Excitation Optical Platform The BD LSRFortessa X-20 optical layout allows for up to five lasers.* Laser Power 355 nm: 15 mW 405 nm: 50 mW 488 nm: 50 mW 561 nm: 50 mW 640 nm: 40 mW Optical Efficiency Power loss at flow cell: <20% of specified laser power Flow Cell Design Rectangular quartz cuvette:Internal cross-section, 430 x 180 μm External quartz cuvette surfaces are anti-reflective coated for optimal transmission of laser light. Fixed optical assembly with spatially separated laser beams. Emission Optics Optical Coupling The quartz cuvette flow cell is gel-coupled by refractive index-matching optical gel to the fluorescence objective lens (1.2 NA) for optimal collection efficiency. Forward Scatter Detection Photodiode detector with a 488/10 bandpass (BP) filter Side Scatter Detector Photomultiplier tube (PMT) with a 488/10 BP filter Emission Optical Design Emitted light from the gel-coupled cuvette is delivered by fiber optics to the detector arrays. The BD LSRFortessa X-20 uses configurable polygonshaped optical pathways that use signal reflection to maximize signal detection. Please see the filter guide at bdbiosciences.com for information on dye and filter options.

Performance Fluorescence Sensitivity FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC) PE: 30 molecules of equivalent soluble fluorochrome (MESF-PE) PE-Cy™5: 10 molecules of equivalent soluble fluorochrome (MESF-PE Cy5) APC: 70 molecules of equivalent soluble fluorochrome (MESF-APC) FITC and PE measurements performed using SPHERO™ Rainbow Calibration Particles (RCP-30-5A) PE-Cy5 and APC measurements performed using SPHERO™ Ultra Rainbow Calibration Particles (URCP- 38-2K) Fluorescence Resolution Coefficient of variation PI: Area of <3%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN) Fluorescence Linearity Doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488-nm blue laser Forward and Side Scatter Sensitivity Enables separation of fixed platelets from noise. Forward and Side Scatter Resolution Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes. Side Scatter Resolution Enables separation of 0.5-μm beads from noise. Forward Scatter PMT Option A forward scatter PMT upgrade is available for small particle detection. Requires the 100-mW, 488-nm blue laser. Data Acquisition Rate 40,000 events/sec with beads

Fluidics Sample Flow Rates Front button panel provides three modes: RUN, STANDBY, and PRIME Continuously adjustable flow rate, plus three preset flow rates: LO: 12 μL/min MED: 35 μL/min HI: 60 μL/min Standard Fluidic Reservoirs One 8-L sheath container and one 10-L waste container provided. Recommended Fluidics Option BD FACSFlow™ supply system: automated fluidics system, which includes a rolling cart and two 20-L CubitainerR packages

Upgrades

Laser Upgrade package including software upgrade

Future Upgrades

Item ID #1569.

Last Updated: 7th April, 2017