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LSRFortessa cell analyzer

MANUFACTURER Becton Dickinson (manufacturer's website)
CONTACT 1 Debra Jones
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Description

An affordable choice to fit most flow cytometry analyzer needs, the BD LSRFortessa can be ordered with up to 4 lasers—blue, red, violet, and UV—which provides a flexibility in laser wavelengths so assay design can be optimized using the latest fluorescent dyes and substrates. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today’s assay requirement.

Specification

Optics Excitation optics Excitation optical platform The BD LSRFortessa optical layout allows for up to four* lasers. Laser power 355 nm: 20 mW 405 nm: 50 mW 488 nm: 50 mW 640 nm: 40 mW Optical efficiency Power loss at flow cell: <20% of specified laser power Flow cell design Rectangular quartz cuvette: Internal cross-section, 430 x 180 μm External quartz cuvette surfaces are anti-reflective coated for optimal transmission of laser light. Fixed optical assembly with spatially separated laser beams. Emission optics Optical coupling The quartz cuvette flow cell is gel- coupled by refractive index-matching optical gel to the fluorescence objective lens (1.2 NA) for optimal collection efficiency. Forward scatter detection Photodiode detector with a 488/10 bandpass filter Side scatter detector Photomultiplier tube (PMT) with a 488/10 bandpass filter Emission optical design Emitted light from the gel-coupled cuvette is delivered by fiber optics to the detector arrays. The BD LSRFortessa uses BD’s patented octagon- and trigon-shaped optical pathways that use signal reflection to maximize signal detection. Please see the separate filter guide for information on dye and filter options.

Performance Fluorescence sensitivity FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC) PE: 30 molecules of equivalent soluble fluorochrome (MESF-PE) PE-Cy™5: 10 molecules of equivalent soluble fluorochrome (MESF-PE-Cy5) APC: 70 molecules of equivalent soluble fluorochrome (MESF-APC) FITC and PE measurements performed using SPHERO™ Rainbow Calibration Particles (RCP-30-5A) PE-Cy5 and APC measurements performed using SPHERO Ultra Rainbow Calibration Particles (URCP-38-2K) Fluorescence resolution Coefficient of variation PI: Area of <3%, full G0/ G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN) Fluorescence linearity Doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488-nm blue laser Forward and side scatter sensitivity Enables separation of fixed platelets from noise. Forward and side scatter resolution Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes. Side scatter resolution Enables separation of 0.5-μm beads from noise. Forward scatter PMT option A forward scatter PMT upgrade is available for small particle detection through the special order program. Data acquisition rate 40,000 events/s with beads.

Fluidics Sample flow rates Front key panel provides three modes: RUN, STANDBY, and PRIME Continuously adjustable flow rate, plus three preset flow rates: LO: 12 μL/min MED: 35 μL/min HI: 60 μL/min Standard fluidic reservoirs One 8-L sheath container and one 10-L waste container provided. Recommended fluidics option BD FACSFlow™ supply system: automated fluidics system, which includes a rolling cart and two 20-L Cubitainer® packages

Upgrades

Laser Upgrade package including software upgrade

Future Upgrades

Item ID #1568.

Last Updated: 7th April, 2017